Measurement of Intracellular pH of Skeletal Muscle with pH-sensitive Glass Microelectrodes*
نویسندگان
چکیده
منابع مشابه
Measurement of intracellular pH of skeletal muscle with pH-sensitive glass microelectrodes.
We used three methods to examine the relationship among intracellular pH, transmembrane potential, and extracellular pH. Single-barreled electrodes permitted the determination of resting potential and intracellular pH with a minimum of cellular injury. Double-barreled electrodes, which incorporated a reference as well as a pH-sensitive electrode in a single tip, facilitated the direct measureme...
متن کاملThe production and testing of double-barreled pH glass microelectrodes for measurement of intratubular pH.
It lhas been possible to miniatuirize the glass pH electrode to an extent tllat it can be uised to measture the intraltiminal pH of single mammalian nephrons(l). There are, however, a nutmber of problems that arise from the miniaturization process. Thlese includ(le electrode insulation, the incorporation of a suitable reference electrode witlh the pH glass electrode, an(d the ultimate achievabl...
متن کاملCHRISTOF J. SCHWIENING Measurement of intracellular pH: a comparison between ion-sensitive microelectrodes and fluorescent dyes
Intracellular pH (pHi) has been studied extensively for over a century using a wide range of techniques. These techniques have been subject to constant improvements to the extent that useful measurements can now be made in even the smallest of cells. This chapter outlines the development of ion-sensitive microelectrodes and dyes and highlights some of the difficulties and dangers of both of the...
متن کاملECS, intracellular pH, and electrolytes of cardiac and skeletal muscle.
The extracellular space (ECS) of muscle from each ventricle of the heart (RV and LV), the atria, diaphragm, and quadriceps was estimated in the anesthetized rabbit from the distribution volumes of [14C]insulin, [14C]sucrose, [51Cr]EDTA, and C1--. Whole-tissue electrolytes were measured and intracellular electrolytes calculated. The ECS of the tissues varied, increasing in the order quadriceps l...
متن کاملIntracellular pH and Inorganic Phosphate Effects on Skeletal Muscle Force
121 used as stubstituents on Ci and C2; namely H, CH 3 , C 2 H 5 , and CeHs. They are differentiated to have approximately 1, 2, 3, and 5 A in their diameters as spherical models. For simplified discussion, we consider that 2 has bulkier substituents on Ci than on C2. Thus the C2 site will be more reactive than the Ci site for dimerization. In this occasion discussion will be rather limited to ...
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ژورنال
عنوان ژورنال: Journal of Clinical Investigation
سال: 1967
ISSN: 0021-9738
DOI: 10.1172/jci105598